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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by <t>Tukey</t> post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.
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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by <t>Tukey</t> post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.
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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by <t>Tukey</t> post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.
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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by <t>Tukey</t> post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.
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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by <t>Tukey</t> post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.
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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by <t>Tukey</t> post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.
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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by <t>Tukey</t> post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.
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Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by Tukey post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.

Journal: Journal of Neuroscience Methods

Article Title: Brain tissue stiffness is a sensitive marker for acidosis

doi: 10.1016/j.jneumeth.2016.07.002

Figure Lengend Snippet: Respiratory acidosis leads to a stiffening of grey matter. (A) pH in the brain of animals culled by CO 2 overdose, decapitation and barbiturate overdose for five minutes post mortem . CO 2 overdose leads to acidosis in the brain. (B) Normalized apparent elastic modulus of different cerebellar regions after decapitation with and without subsequent incubation at pH 6, amiloride treatment as well as after CO 2 euthanasia. Numbers of animals (top) and numbers of measurements (bottom) are shown on the bars, significance levels are indicated above the bars (one-way ANOVA followed by Tukey post-hoc test). Error bars show the standard deviation and indicate inter-animal variability.

Article Snippet: The averages of the median values of each animal were calculated and significance tested using One Way or Two Way ANOVA tests, followed by a post-hoc Tukey test (OriginPro 8.5, OriginLab Corporation, Northhampton, USA).

Techniques: Incubation, Standard Deviation